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Sample GSM4038301 Query DataSets for GSM4038301
Status Public on Aug 20, 2019
Title Salivary supernatant from healthy control, biological rep1
Sample type RNA
 
Source name Salivary supernatant from healthy control
Organism Homo sapiens
Characteristics disease state: control
gender: Female
sample type: Salivary supernatant
Treatment protocol All samples were centrifuged at 3000 × g for 15 minutes at 4°C to get rid of the cells. Only the supernatant was transferred into a 2 mL centrifuge tube. Then, to completely remove the cellular components, a further centrifugation of 12000 × g for 10 minutes at 4°C was followed. The supernatant was transferred into a 2 mL centrifuge tube and stored at -80°C until use.
Growth protocol Before saliva collection, all patients and healthy controls were required to avoid from eating, drinking or smoking for at least 2 hours. At least 3mL saliva was collected from each subject.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (FlashTag™ Biotin HSR RNA Labeling Kit, Affymetrix).
 
Hybridization protocol 21.5 μL biotin-labeled sample were hybridized for 16 hr at 48℃ on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned according to the standard Affymetrix protocol. (Affymetrix® Command Console® User Manual (P/N 702569))
Description Gene expression data from salivary supernatant of MPE patients and healthy controls
Control1
Data processing Use Expression Console software for data summarization, normalization, and quality control.
 
Submission date Aug 19, 2019
Last update date Aug 21, 2019
Contact name Yuan Yang
Organization name Beijing Chaoyang Hospital, Capital Medical University
Street address 8 Gongti Nanlu, Chaoyang District
City Beijing
ZIP/Postal code 100020
Country China
 
Platform ID GPL19117
Series (1)
GSE135947 Salivary microRNA expression data from malignant pleural effusion patients and healthy controls

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
MIMAT0000001_st 0.516231172
MIMAT0015091_st 0.040295464
MIMAT0000002_st 0.507655027
MIMAT0015092_st 0.51068287
MIMAT0020301_st 0.172682428
MIMAT0000003_st 0.533748602
MIMAT0020302_st 0.076211437
MIMAT0000004_st 0.38054759
MIMAT0000005_st 0.265805679
MIMAT0015093_st 0.280388926
MIMAT0020303_st 0.081374348
MIMAT0000006_st 0.308150824
MIMAT0020304_st 0.315178732
MIMAT0000007_st -0.104632235
MIMAT0015094_st 0.086782709
MIMAT0000008_st 0.367460575
MIMAT0020305_st 0.159298322
MIMAT0000009_st 0.048713713
MIMAT0020306_st 0.165802953
MIMAT0000010_st 0.331641855

Total number of rows: 36353

Table truncated, full table size 985 Kbytes.




Supplementary file Size Download File type/resource
GSM4038301_Control1_miRNA-4_0_.CEL.gz 607.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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