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Status |
Public on Aug 20, 2019 |
Title |
Salivary supernatant from MPE patient, biological rep1 |
Sample type |
RNA |
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Source name |
Salivary supernatant from MPE patient
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Organism |
Homo sapiens |
Characteristics |
disease state: Malignant pleural effusion (MPE) gender: Female sample type: Salivary supernatant
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Treatment protocol |
All samples were centrifuged at 3000 × g for 15 minutes at 4°C to get rid of the cells. Only the supernatant was transferred into a 2 mL centrifuge tube. Then, to completely remove the cellular components, a further centrifugation of 12000 × g for 10 minutes at 4°C was followed. The supernatant was transferred into a 2 mL centrifuge tube and stored at -80°C until use.
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Growth protocol |
Before saliva collection, all patients and healthy controls were required to avoid from eating, drinking or smoking for at least 2 hours. At least 3mL saliva was collected from each subject.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (FlashTag™ Biotin HSR RNA Labeling Kit, Affymetrix).
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Hybridization protocol |
21.5 μL biotin-labeled sample were hybridized for 16 hr at 48℃ on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol. (Affymetrix® Command Console® User Manual (P/N 702569))
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Description |
Gene expression data from salivary supernatant of MPE patients and healthy controls MPE1
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Data processing |
Use Expression Console software for data summarization, normalization, and quality control.
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Submission date |
Aug 19, 2019 |
Last update date |
Aug 21, 2019 |
Contact name |
Yuan Yang |
Organization name |
Beijing Chaoyang Hospital, Capital Medical University
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Street address |
8 Gongti Nanlu, Chaoyang District
|
City |
Beijing |
ZIP/Postal code |
100020 |
Country |
China |
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Platform ID |
GPL19117 |
Series (1) |
GSE135947 |
Salivary microRNA expression data from malignant pleural effusion patients and healthy controls |
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