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Sample GSM4026070 Query DataSets for GSM4026070
Status Public on Aug 07, 2023
Title C354_MCM (S787Nr17)
Sample type SRA
Source name primary pancreatic tumor line
Organism Homo sapiens
Characteristics cell line: primary pancreatic tumor line, C354
treatment: Macrophage conditioned media
Treatment protocol For experiments, cells were seeded in DMEM/F12 supplemented with 0.2% B27 anf FGFb in the presence or absence of macrophage-conditioned medium or 20 uM etomoxir
Growth protocol Cells were maintained in RPMI with 10% FBS
Extracted molecule total RNA
Extraction protocol Trizol extraction following manufacturer´s protocol
RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq Stranded mRNA)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description S787Nr17
Data processing Demultiplexing of the sequencing reads was performed with Illumina bcl2fastq
Adapters were trimmed with Skewer (version 0.2.2, Jiang et al. 2014).
The quality of FASTQ files was analyzed with FastQC (version 0.11.5, Andrews 2010).
Reads were aligned using STAR (version 2.5.3a)
Reads count were performed by HT-seq using annotation from gencode.genome.v27
Genome_build: human genome build 38
Supplementary_files_format_and_content: tab-delimited text file for raw read counts for each Sample
Submission date Aug 10, 2019
Last update date Aug 07, 2023
Contact name Patricia Sancho
Organization name Hospital Miguel Servet IIS Aragón
Department Traslational Research Unit
Lab Metabolism & Cancer Stem Cells
Street address P. Isabel la Católica 1-3
City Zaragoza
State/province Aragón
ZIP/Postal code 50009
Country Spain
Platform ID GPL24676
Series (1)
GSE135686 Gene-expression profiling in pancreatic cancer cells treated with macrophage conditioned media and etomoxir
BioSample SAMN12549535
SRA SRX6693470

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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