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Sample GSM4007709 Query DataSets for GSM4007709
Status Public on Aug 01, 2022
Title G3 2
Sample type SRA
Source name left ventricular tissue
Organism Mus musculus
Characteristics genotype/variation: G3
strain: C57BL/6J
tissue: heart
age: 12m
Extracted molecule total RNA
Extraction protocol Hearts were removed, flash frozen using liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
Description CG3_67
Data processing Illumina Casava software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
FPKM (expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced) were calculated using a protocol from Trapnell, et al., Nature Biotechnology, 2010.
Genome_build: mm9
Supplementary_files_format_and_content: FPKM and counts
Submission date Aug 05, 2019
Last update date Aug 01, 2022
Contact name Xudong Zhu
Organization name Hangzhou Normal University
Street address #2318 Yuhangtang Road
City Hangzhou
ZIP/Postal code 311121
Country China
Platform ID GPL21273
Series (2)
GSE135404 Fine-Tuning of PGC1α Expression Regulates Cardiac Function and Longevity (RNA-seq: telomerase-deficient vs PGC1aOE)
GSE136787 Fine-Tuning of PGC1α Expression Regulates Cardiac Function and Longevity
BioSample SAMN12497558
SRA SRX6657694

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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