|Public on Jun 30, 2021
|cell line: NOMO1
cell tye: AML cell line
|Cells were plated 24 hours before treatment with either DMSO, GSK3484862, GSK3685032, DAC, or GSK3510477 then grown for the indicated time before collection.
|Cell lines were obtained from the American Type Culture Collection, the Deutsche Sammlung von Mikroorganismen und Zellbulturen, or AddexBio and maintained in the recommended cell culture media at 37°C in 5% CO2.
|RNA was extracted using Qiagen Rneasy Mini kit
Libraries were prepared at the NYU Medical Center Genome Technology Center
All 150 PE libraries were sequenced across 6 lanes for a total of 12 fastq samples per sample.
|Illumina NovaSeq 6000
|processed data file: DNMT1_AML_fpkm_NOMO1_table.txt
|Salmon_v0.12.0 transcript quantification
DEseq2 log2 fold change differential expression and normalized fpkm quantification
fGSEA gene set enrichment analysis
Genome_build: Human hg38
Supplementary_files_format_and_content: fpkm values for all quantifiable genes; log2 fold change values for all quantifiable genes
|Aug 05, 2019
|Last update date
|Jun 30, 2021
|Shawn W Foley
|1250 S. Collegeville Rd
|Expression profiling and methylation analysis of DNMT1 inhibitor treated AML cell lines
|RNA-seq of DNMT1 inhibitor treated AML cell lines