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Sample GSM399335 Query DataSets for GSM399335
Status Public on Apr 30, 2012
Title NA10856
Sample type RNA
Source name lymphoblastoid cell line
Organism Homo sapiens
Characteristics cell line: lymphoblastoid cell line
parent/child: HapMap CEU_children
coriell sample: NA10856
Biomaterial provider Coriell sample NA10856
Growth protocol RPMI 1640 with Glutamax I medium (Invitrogen Corporation) supplemented with 10% fetal calf serum and 1% penicillin and streptomycin mix (Invitrogen Corporation). Cells lines were harvested at a density of 0.6 ~ 1 x 10^6 cells/ml and at least 80 % viability. Cultures were spun for 5 min at 1000 g, and the resulting pellets were washed once in PBS and lysed by adding 2 ml of micro glass beads (Sigma) and vortexing in 1 ml lysis solution containing beta-mercaptoethanol (Qiagen, RNeasy kit). Cell lysates were stored at -80°C.
Extracted molecule total RNA
Extraction protocol RNAs were extracted using RNeasy mini kits with on-column DNAse I digestion (Qiagen)
Label Cy5
Label protocol 1.5 ug of total RNA from each sample was used in cDNA synthesis and IVT reaction using the Amino Allyl MessageAmpTMII aRNA Amplification kit (Ambion, Austin, TX 78744-1832, cat. no.1753). Single stranded aRNA labeled with Cy5 was generated using the Cy5 Mono-Reactive dye pack (Amersham. PA25001)
Hybridization protocol Ten micrograms of Cy5- labeled aRNA was fragmented (Ambion cat# 8740) and hybridized 16 hrs to Phalanx HOA arrays according to manufacturer’s instructions
Scan protocol Arrays were scanned using Agilent DNA Microarray Scanner G2565B (Agilent Technologies, Inc. CA, USA) at settings of 60 PMT (for Cy5) and of 10 micron, and the resulting images were quantified using the software ProbeArrayer (an in-house tool developed by ITRI)
Description no additional information
Data processing log2 transformed intensities were normalized across technical replicates for each individual using a ComBat (Johnson et al. 2007) normalization. The normalized replicate values were merged by median as one chip per sample, and then sample-based chips were normalized by median across individuals (n=60 for parent or n=30 for children).
Submission date Apr 30, 2009
Last update date Apr 30, 2012
Contact name Li-Lan Li
Organization name Industrial Technology Research Institute
Street address 195,Sec. 4, Chung Hsing Rd. Chutung
City Hsinchu
ZIP/Postal code 310
Country Taiwan
Platform ID GPL6254
Series (1)
GSE15905 A new genome wide expression array and its application to eQTL mapping, synergy to other platforms

Data table header descriptions
VALUE Normalized, log2 signal intensity

Data table
PH_hs_0004877 6.670996885
PH_hs_0033433 6.907568826
PH_hs_0026582 5.938469603
PH_hs_0035481 14.03240449
PH_hs_0025134 7.881673603
PH_hs_0023499 12.6459737
PH_hs_0025293 6.145003116
PH_hs_0023725 9.504142943
PH_hs_0032109 8.396079813
PH_hs_0010306 8.015177405
PH_hs_0001352 6.976233488
PH_hs_0022361 8.081553138
PH_hs_0014165 11.93817698
PH_hs_0032993 4.379837865
PH_hs_0021034 14.22902383
PH_hs_0011065 7.64993283
PH_hs_0041074 5.725665054
PH_hs_0004580 10.82265797
PH_hs_0000364 7.510001852
PH_hs_0012615 6.190519645

Total number of rows: 30968

Table truncated, full table size 776 Kbytes.

Supplementary file Size Download File type/resource
GSM399335_H0601018018.txt.gz 344.3 Kb (ftp)(http) TXT
GSM399335_H0601018021.txt.gz 346.5 Kb (ftp)(http) TXT
GSM399335_H0601018022.txt.gz 345.7 Kb (ftp)(http) TXT
GSM399335_H0601018030.txt.gz 345.5 Kb (ftp)(http) TXT
Processed data included within Sample table

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