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Sample GSM399331 Query DataSets for GSM399331
Status Public on Apr 30, 2012
Title NA10847
Sample type RNA
 
Source name lymphoblastoid cell line
Organism Homo sapiens
Characteristics cell line: lymphoblastoid cell line
parent/child: HapMap CEU_children
coriell sample: NA10847
Biomaterial provider Coriell sample NA10847
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA10847
Growth protocol RPMI 1640 with Glutamax I medium (Invitrogen Corporation) supplemented with 10% fetal calf serum and 1% penicillin and streptomycin mix (Invitrogen Corporation). Cells lines were harvested at a density of 0.6 ~ 1 x 10^6 cells/ml and at least 80 % viability. Cultures were spun for 5 min at 1000 g, and the resulting pellets were washed once in PBS and lysed by adding 2 ml of micro glass beads (Sigma) and vortexing in 1 ml lysis solution containing beta-mercaptoethanol (Qiagen, RNeasy kit). Cell lysates were stored at -80°C.
Extracted molecule total RNA
Extraction protocol RNAs were extracted using RNeasy mini kits with on-column DNAse I digestion (Qiagen)
Label Cy5
Label protocol 1.5 ug of total RNA from each sample was used in cDNA synthesis and IVT reaction using the Amino Allyl MessageAmpTMII aRNA Amplification kit (Ambion, Austin, TX 78744-1832, cat. no.1753). Single stranded aRNA labeled with Cy5 was generated using the Cy5 Mono-Reactive dye pack (Amersham. PA25001)
 
Hybridization protocol Ten micrograms of Cy5- labeled aRNA was fragmented (Ambion cat# 8740) and hybridized 16 hrs to Phalanx HOA arrays according to manufacturer’s instructions
Scan protocol Arrays were scanned using Agilent DNA Microarray Scanner G2565B (Agilent Technologies, Inc. CA, USA) at settings of 60 PMT (for Cy5) and of 10 micron, and the resulting images were quantified using the software ProbeArrayer (an in-house tool developed by ITRI)
Description no additional information
Data processing log2 transformed intensities were normalized across technical replicates for each individual using a ComBat (Johnson et al. 2007) normalization. The normalized replicate values were merged by median as one chip per sample, and then sample-based chips were normalized by median across individuals (n=60 for parent or n=30 for children).
 
Submission date Apr 30, 2009
Last update date Apr 30, 2012
Contact name Li-Lan Li
E-mail(s) lilanli@itri.org.tw
Organization name Industrial Technology Research Institute
Street address 195,Sec. 4, Chung Hsing Rd. Chutung
City Hsinchu
ZIP/Postal code 310
Country Taiwan
 
Platform ID GPL6254
Series (1)
GSE15905 A new genome wide expression array and its application to eQTL mapping, synergy to other platforms

Data table header descriptions
ID_REF
VALUE Normalized, log2 signal intensity

Data table
ID_REF VALUE
PH_hs_0004877 7.310728892
PH_hs_0033433 6.747483535
PH_hs_0026582 5.567428855
PH_hs_0035481 13.76320583
PH_hs_0025134 8.08486455
PH_hs_0023499 13.06135721
PH_hs_0025293 6.816030602
PH_hs_0023725 9.63575088
PH_hs_0032109 9.66221183
PH_hs_0010306 8.710813887
PH_hs_0001352 7.261475491
PH_hs_0022361 8.284894796
PH_hs_0014165 13.24060948
PH_hs_0032993 3.354736587
PH_hs_0021034 14.52187234
PH_hs_0011065 7.752507273
PH_hs_0041074 3.433508769
PH_hs_0004580 8.969055351
PH_hs_0000364 7.550445365
PH_hs_0012615 6.160327469

Total number of rows: 30968

Table truncated, full table size 778 Kbytes.




Supplementary file Size Download File type/resource
GSM399331_H0601017779.txt.gz 349.2 Kb (ftp)(http) TXT
GSM399331_H0601017780.txt.gz 345.1 Kb (ftp)(http) TXT
GSM399331_H0601017802.txt.gz 345.6 Kb (ftp)(http) TXT
GSM399331_H0601017996.txt.gz 348.5 Kb (ftp)(http) TXT
GSM399331_H0601017997.txt.gz 345.6 Kb (ftp)(http) TXT
GSM399331_H0601018003.txt.gz 341.7 Kb (ftp)(http) TXT
Processed data included within Sample table

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