|
| Status |
Public on Nov 04, 2019 |
| Title |
Wildtype liver tissue [OGT_WT_4] |
| Sample type |
SRA |
| |
|
| Source name |
Liver tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
genotype/variation: Wildtype
tissue: Liver
age: 5w
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver tissue with TRIzol reagent according to the manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequencing reads were aligned against the mouse mm10 reference genome using the TopHat2 software version 2.1.0 with the default parameters.
Mapped reads were assigned to gene features and quantified using the HTSeq software version 0.9.1.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: *.txt: Tab-delimited text files include HTSeq raw abundance values for each sample.
|
| |
|
| Submission date |
Jul 28, 2019 |
| Last update date |
Nov 04, 2019 |
| Contact name |
Xiaoyong Yang |
| E-mail(s) |
xiaoyong.yang@yale.edu
|
| Organization name |
Yale University
|
| Department |
Comparative Medicine
|
| Street address |
375 Congress Ave
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520-8016 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE134993 |
Genome-wide changes in OGT-deficient mouse liver tissue |
|
| Relations |
| BioSample |
SAMN12391861 |
| SRA |
SRX6610150 |
