|
Status |
Public on Apr 28, 2009 |
Title |
GBM_Cell Line_5 |
Sample type |
RNA |
|
|
Source name |
GBM
|
Organism |
Homo sapiens |
Characteristics |
cell line: GBM
|
Treatment protocol |
Full details of the cell lines culture prior RNA extraction have been described by Fael Al-Mayhani et al, 2009
|
Growth protocol |
GBM cell lines were derived from excised GBM tumours and cultured under serum-free conditions with EGF and FGF. Full details of the derivation and culture protocol have been described by Fael Al-Mayhani et al, 2009
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Qiagen RNAeasy kit
|
Label |
Cy3
|
Label protocol |
Cy3
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol by Centre for Microarray Resources at the department of Pathology of the University of Cambridge, UK
|
Scan protocol |
Standard Illumina scan protocol by Centre for Microarray Resources at the department of Pathology of the University of Cambridge, UK
|
Description |
none
|
Data processing |
Robust spline normalization
|
|
|
Submission date |
Apr 27, 2009 |
Last update date |
Apr 27, 2009 |
Contact name |
Talal M. Fael Al-Mayhani |
Phone |
01223-331160
|
Fax |
01223-331174
|
Organization name |
University of Cambridge
|
Department |
Clinical Neurosciences
|
Street address |
Forvie Site, Robinson Way
|
City |
Cambridge |
State/province |
Cambridgeshire |
ZIP/Postal code |
CB2 0PY |
Country |
United Kingdom |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE15846 |
GBM cell lines derived and propagated according to Cambridge protocol |
|