|
| Status |
Public on Apr 28, 2009 |
| Title |
GBM_Cell Line_4 |
| Sample type |
RNA |
| |
|
| Source name |
GBM
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GBM
|
| Treatment protocol |
Full details of the cell lines culture prior RNA extraction have been described by Fael Al-Mayhani et al, 2009
|
| Growth protocol |
GBM cell lines were derived from excised GBM tumours and cultured under serum-free conditions with EGF and FGF. Full details of the derivation and culture protocol have been described by Fael Al-Mayhani et al, 2009
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNAeasy kit
|
| Label |
Cy3
|
| Label protocol |
Cy3
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol by Centre for Microarray Resources at the department of Pathology of the University of Cambridge, UK
|
| Scan protocol |
Standard Illumina scan protocol by Centre for Microarray Resources at the department of Pathology of the University of Cambridge, UK
|
| Description |
none
|
| Data processing |
Robust spline normalization
|
| |
|
| Submission date |
Apr 27, 2009 |
| Last update date |
Apr 27, 2009 |
| Contact name |
Talal M. Fael Al-Mayhani |
| Phone |
01223-331160
|
| Fax |
01223-331174
|
| Organization name |
University of Cambridge
|
| Department |
Clinical Neurosciences
|
| Street address |
Forvie Site, Robinson Way
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0PY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE15846 |
GBM cell lines derived and propagated according to Cambridge protocol |
|
