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Sample GSM3978656 Query DataSets for GSM3978656
Status Public on Aug 19, 2019
Title MatureCD4SP
Sample type SRA
 
Source name sorted thymocytes
Organism Mus musculus
Characteristics cell type: mature CD25- Foxp3- CD4SP thymocytes
strain: Foxp3-GFP
Growth protocol Thymus was dissociated by mechanical disruption. CD8 expressing cells were depleted and populations of interest were purified by FACS sorting.
Extracted molecule polyA RNA
Extraction protocol 10X Genomics protocols (Chromium Single Cell 3’ Reagent Kits v2 Chemistry)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description MatureCD4SP
Data processing single-cell RNA-seq data was processed from raw fastq files to count matrices with SEQC (see https://github.com/ambrosejcarr/seqc.git)
Genome_build: mm10 from ENSEMBL GRCm38
Supplementary_files_format_and_content: Cell x gene unnormalized count matrices in gzipped csv format
 
Submission date Jul 25, 2019
Last update date Aug 20, 2019
Contact name Elham Azizi
E-mail(s) elham.azizi@gmail.com
Organization name Memorial Sloan Kettering Cancer Center
Street address 417 E 68th St Z1141
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL24247
Series (1)
GSE134902 scRNAseq transcriptome analysis of developing regulatory T cells and precursors in the thymus
Relations
BioSample SAMN12367436
SRA SRX6595735

Supplementary file Size Download File type/resource
GSM3978656_MatureCD4SP_dense.csv.gz 8.3 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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