|
Status |
Public on Aug 19, 2019 |
Title |
Foxp3plus |
Sample type |
SRA |
|
|
Source name |
sorted thymocytes
|
Organism |
Mus musculus |
Characteristics |
cell type: Foxp3+ CD4SP thymocytes strain: Foxp3-GFP
|
Growth protocol |
Thymus was dissociated by mechanical disruption. CD8 expressing cells were depleted and populations of interest were purified by FACS sorting.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
10X Genomics protocols (Chromium Single Cell 3’ Reagent Kits v2 Chemistry)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Foxp3plus
|
Data processing |
single-cell RNA-seq data was processed from raw fastq files to count matrices with SEQC (see https://github.com/ambrosejcarr/seqc.git) Genome_build: mm10 from ENSEMBL GRCm38 Supplementary_files_format_and_content: Cell x gene unnormalized count matrices in gzipped csv format
|
|
|
Submission date |
Jul 25, 2019 |
Last update date |
Aug 20, 2019 |
Contact name |
Elham Azizi |
E-mail(s) |
elham.azizi@gmail.com
|
Organization name |
Memorial Sloan Kettering Cancer Center
|
Street address |
417 E 68th St Z1141
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE134902 |
scRNAseq transcriptome analysis of developing regulatory T cells and precursors in the thymus |
|
Relations |
BioSample |
SAMN12367437 |
SRA |
SRX6595734 |