|
Status |
Public on Aug 08, 2019 |
Title |
Pcy_PBS_12 |
Sample type |
SRA |
|
|
Source name |
kidney
|
Organism |
Mus musculus |
Characteristics |
tissue: kidney treatment: PBS dose: 0mpk treatment_duration: weekly strain: Pcy/CD1
|
Treatment protocol |
Five-weeks-old Pcy/CD1 mice were treated with subcutaneous injections of either PBS or RGLS4326 at 25 mg kg-1 monthly for 25-weeks. Five age-matched untreated male WT/CD1 mice were included for comparison
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Kidneys were removed, flash frozen on dry ice, and RNA was harvested using Qiagen RNeasy kit TruSeq Stranded mRNA Library Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Data was collected using the NextSeq 500 sequencer per manufacturer's protocol (Illumina). Sequencing quality was assessed with the FastQC tool v0.11.2 and trimmed with TrimGalore. Reads were mapped using STAR with samtools v1.1. HTSeq v0.6.1 was used to generate counts. Map and count quality was assessed by picard tools v1.119 and RSeQC tool. Genome_build: GRCm38 (mm10) Supplementary_files_format_and_content: Raw counts per gene
|
|
|
Submission date |
Jul 23, 2019 |
Last update date |
Aug 08, 2019 |
Contact name |
Sole Gatto |
E-mail(s) |
sole.gattov1.0@gmail.com
|
Organization name |
Monoceros Biosystems
|
Street address |
12636 High Bluff Drive, Suite 400
|
City |
San Diego |
State/province |
CA |
ZIP/Postal code |
92130 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE134718 |
Transcriptomic profile of Pcy mice treated with RGLS4326 |
GSE134721 |
Transcriptomic profiles of Pcy and Pdk2KO mice and human kidney epithelial cells treated with RGLS4326 |
|
Relations |
BioSample |
SAMN12343220 |
SRA |
SRX6491493 |