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Sample GSM3964106 Query DataSets for GSM3964106
Status Public on Aug 08, 2019
Title Pcy_PBS_12
Sample type SRA
 
Source name kidney
Organism Mus musculus
Characteristics tissue: kidney
treatment: PBS
dose: 0mpk
treatment_duration: weekly
strain: Pcy/CD1
Treatment protocol Five-weeks-old Pcy/CD1 mice were treated with subcutaneous injections of either PBS or RGLS4326 at 25 mg kg-1 monthly for 25-weeks. Five age-matched untreated male WT/CD1 mice were included for comparison
Extracted molecule polyA RNA
Extraction protocol Kidneys were removed, flash frozen on dry ice, and RNA was harvested using Qiagen RNeasy kit
TruSeq Stranded mRNA Library Prep Kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Data was collected using the NextSeq 500 sequencer per manufacturer's protocol (Illumina).
Sequencing quality was assessed with the FastQC tool v0.11.2 and trimmed with TrimGalore. Reads were mapped using STAR with samtools v1.1.
HTSeq v0.6.1 was used to generate counts. Map and count quality was assessed by picard tools v1.119 and RSeQC tool.
Genome_build: GRCm38 (mm10)
Supplementary_files_format_and_content: Raw counts per gene
 
Submission date Jul 23, 2019
Last update date Aug 08, 2019
Contact name Sole Gatto
E-mail(s) sole.gattov1.0@gmail.com
Organization name Monoceros Biosystems
Street address 12636 High Bluff Drive, Suite 400
City San Diego
State/province CA
ZIP/Postal code 92130
Country USA
 
Platform ID GPL19057
Series (2)
GSE134718 Transcriptomic profile of Pcy mice treated with RGLS4326
GSE134721 Transcriptomic profiles of Pcy and Pdk2KO mice and human kidney epithelial cells treated with RGLS4326
Relations
BioSample SAMN12343220
SRA SRX6491493

Supplementary file Size Download File type/resource
GSM3964106_RS001900129_S4_LallAligned_genecounts.txt.gz 98.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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