|
| Status |
Public on Jul 01, 2020 |
| Title |
Whole seedlings, H2O2.wt.rep2 |
| Sample type |
SRA |
| |
|
| Source name |
H2O2.wt
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: whole seedlings
age: 14-day-old
genotype: wt
treatment: H2O2
|
| Treatment protocol |
Lowlight treatment: seedlings of wildtype and bzip68 mutant were grown on 1/2 MS plates in presence of white light (30 umoles/m2 seg). Mock: seedlings of wildtype and bzip68 mutant were grown on 1/2 MS plates in presence of white light (125 umoles/m2 seg) for 14 days then sparyed with water supplied with 0.01% triton X-100. H2O2 treatment: seedlings of wildtype and bzip68 mutant were grown on 1/2 MS plates in presence of white light (125 umoles/m2 seg) for 14 days then sparyed with 5 mM H2O2 supplied with 0.01% triton X-100.
|
| Growth protocol |
Arabidopsis seedlings were growth on 1/2 MS plates containing 3% sucrose and 0.8% agar for 14 days under 16h light/ 8h dark condition(with light 125 umoles/m2 seg )
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Agilent Plant RNA Isolation Mini kit according to the manufacturer’s instructions. Bar-coded mRNA libraries were constructed by BGI (Shenzhen, China) using the Illumina’s reagents and protocols according to the manufacturer’s instruction. Samples were sequenced on Illumina HiSeq 2500 system.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
20190704.bzip.counts.xlsx
|
| Data processing |
TopHat 2.1.0 software (Trapnell, Pachter and Salzberg, 2009) was used to map the reads to the Arabidopsis genome (TAIR10). RSEM 2.1.30 (Anders, Pyl and Huber, 2015) was used for statistical analysis of the read counts for each gene.
Genome_build: A. thaliana Ensemble 41 (TAIR10)
Supplementary_files_format_and_content: 20190704.bzip.counts.xlsx, Supplementary_Tables_S1_S2.BZIP68_CHIPSeq_peaks_v4.xlsx
|
| |
|
| Submission date |
Jul 06, 2019 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Huan ZHONG |
| E-mail(s) |
zhdorothy5@gmail.com
|
| Phone |
91493987
|
| Organization name |
HKBU
|
| Department |
BIOLOGY
|
| Street address |
T1012 CHA CHI-MING SCIENCE TOWER
|
| City |
HONG KONG |
| State/province |
HONG KONG |
| ZIP/Postal code |
00000 |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL17639 |
| Series (1) |
| GSE133900 |
Redox-sensitive bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis |
|
| Relations |
| BioSample |
SAMN12226110 |
| SRA |
SRX6410170 |
