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Sample GSM3912470 Query DataSets for GSM3912470
Status Public on Aug 05, 2020
Title EGA3
Sample type RNA
Source name ob/ob EGA islets, biological replicate 3
Organism Mus musculus
Characteristics tissue: pancreatic islets
strain: C57Bl/6J
gender: male
Treatment protocol Pancreatic islets were isolated from lean (WT) mice or from ob/ob mice, 28 days after sham or EGA surgery.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy kit (Qiagen), according to manufacturer guidelines.
Label Cy3
Label protocol Total RNA from each sample was amplified and transcribed into fluorescent Cyanine-3 (cy3) cRNA using the manufacturer’s Agilent’s Quick Amp Labeling protocol (version 5.7, Agilent Technologies).
Hybridization protocol The labeled cRNAs were hybridized onto the Whole Mouse Genome Oligo Microarray (4 x 44K, Agilent Technologies) based on the manufacturer’s standard protocols.
Scan protocol Slides were scanned immediately after washing on the Agilent Scanner G2505C.
Description Gene expression 28 days after EGA surgery
Data processing Agilent Feature Extraction software (version was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v12.1 software (Agilent Technologies). After quantile normalization of the raw data, genes that at least 3 out of 6 samples have flags in Detected (“All Targets Value”) were chosen for further data analysis.
Submission date Jul 01, 2019
Last update date Aug 05, 2020
Contact name Claudiane Guay
Phone +41216925281
Organization name University of Lausanne
Department DNF
Street address Du Bugnon 9
City Lausanne
State/province Vaud
ZIP/Postal code 1005
Country Switzerland
Platform ID GPL11202
Series (2)
GSE133577 Effects of entero-gastro-anastomosis (EGA) surgery in ob/ob mice on pancreatic islet gene expression
GSE133852 ob/ob mice on pancreatic islet

Data table header descriptions
VALUE log2 value of Normalized Intensity of each sample

Data table
A_55_P1989846 6.022436
A_55_P2022211 8.25808
A_55_P1980764 6.0968485
A_55_P1964375 10.359366
A_51_P128876 8.682472
A_55_P2121042 6.4186645
A_51_P207591 4.2397532
A_55_P2131920 6.4370975
A_55_P2404223 8.034777
A_55_P2101944 16.998201
A_52_P358860 8.455882
A_51_P119031 10.623061
A_51_P309854 7.5296593
A_51_P343900 10.995541
A_51_P234359 5.7189174
A_51_P487813 10.346262
A_52_P613977 9.431858
A_55_P2096028 4.507213
A_55_P2052210 14.069897
A_51_P128987 9.154285

Total number of rows: 30398

Table truncated, full table size 686 Kbytes.

Supplementary file Size Download File type/resource
GSM3912470_EGA3.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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