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Sample GSM3912461 Query DataSets for GSM3912461
Status Public on Aug 05, 2020
Title WT1
Sample type RNA
 
Source name C57Bl/6J islets, biological replicate 1
Organism Mus musculus
Characteristics tissue: pancreatic islets
strain: C57Bl/6J
gender: male
Treatment protocol Pancreatic islets were isolated from lean (WT) mice or from ob/ob mice, 28 days after sham or EGA surgery.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy kit (Qiagen), according to manufacturer guidelines.
Label Cy3
Label protocol Total RNA from each sample was amplified and transcribed into fluorescent Cyanine-3 (cy3) cRNA using the manufacturer’s Agilent’s Quick Amp Labeling protocol (version 5.7, Agilent Technologies).
 
Hybridization protocol The labeled cRNAs were hybridized onto the Whole Mouse Genome Oligo Microarray (4 x 44K, Agilent Technologies) based on the manufacturer’s standard protocols.
Scan protocol Slides were scanned immediately after washing on the Agilent Scanner G2505C.
Description Gene expression in control mice
Data processing Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v12.1 software (Agilent Technologies). After quantile normalization of the raw data, genes that at least 3 out of 6 samples have flags in Detected (“All Targets Value”) were chosen for further data analysis.
 
Submission date Jul 01, 2019
Last update date Aug 05, 2020
Contact name Claudiane Guay
E-mail(s) claudiane.guay@unil.ch
Phone +41216925281
Organization name University of Lausanne
Department DNF
Street address Du Bugnon 9
City Lausanne
State/province Vaud
ZIP/Postal code 1005
Country Switzerland
 
Platform ID GPL11202
Series (2)
GSE133577 Effects of entero-gastro-anastomosis (EGA) surgery in ob/ob mice on pancreatic islet gene expression
GSE133852 ob/ob mice on pancreatic islet

Data table header descriptions
ID_REF
VALUE log2 value of Normalized Intensity of each sample

Data table
ID_REF VALUE
A_55_P1989846 5.764439
A_55_P2022211 8.433135
A_55_P1980764 5.9508867
A_55_P1964375 9.524455
A_51_P128876 9.147289
A_55_P2121042 6.7117276
A_51_P207591 2.7943547
A_55_P2131920 5.5442576
A_55_P2404223 8.0261545
A_55_P2101944 16.659718
A_52_P358860 9.294756
A_51_P119031 10.166202
A_51_P309854 8.107637
A_51_P343900 10.994224
A_51_P234359 6.2375927
A_51_P487813 9.910025
A_52_P613977 9.964312
A_55_P2096028 3.2157822
A_55_P2052210 14.211928
A_51_P128987 8.858454

Total number of rows: 30398

Table truncated, full table size 686 Kbytes.




Supplementary file Size Download File type/resource
GSM3912461_WT1.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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