|
| Status |
Public on Sep 27, 2023 |
| Title |
IF16 rep5 |
| Sample type |
SRA |
| |
|
| Source name |
Heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Heart
age: 9 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole hearts were removed and flash frozen on dry ice before RNA extraction. Frozen tissues were used for total RNA extraction using commercial RNA extraction kit from Bio Basic (EZ-10 DNAaway RNA miniprep kit).
RNA libraries were prepared for sequencing using standard Illumina protocols by NOVOGENE.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Daily intermittent fasting for 16 hours
|
| Data processing |
Indexes of the reference genome were built using Bowtie v2.0.6 and paired-end clean reads were aligned to the reference genome using TopHat v2.0.9.
HTSeq v0.6.1 was used to count the read numbers mapped of each gene.
Genome_build: mm9
Supplementary_files_format_and_content: Excel files containing FPKM values and Readcount values for all samples.
|
| |
|
| Submission date |
Jun 25, 2019 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Joonki Kim |
| E-mail(s) |
need2pro@gmail.com
|
| Organization name |
National University of Singapore
|
| Department |
Physiology, School Of Medicine
|
| Lab |
ESIR
|
| Street address |
2 Medical drive, Blk MD9
|
| City |
Singapore |
| ZIP/Postal code |
117593 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE133290 |
Transcriptome analysis of mouse heart under different intermittent fasting conditions |
|
| Relations |
| BioSample |
SAMN12136663 |
| SRA |
SRX6366279 |
