NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3896355 Query DataSets for GSM3896355
Status Public on Aug 14, 2019
Title Rfx6 fl/+ #18
Sample type RNA
 
Source name Control, replicate 3
Organism Mus musculus
Characteristics condition: Control
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRI-reagent (Invitrogen)
Label Cy3
Label protocol Complementary RNA (cRNA) samples were linearly amplified and labeled with cyanine 3 starting from 100 ng of total RNA (One-Color Microarray-Based Gene Expression Analysis- Low Input Quick Amp Labeling” version 6.5, May 2010, Part Number G4140-90040 protocol)
 
Hybridization protocol Following fragmentation, labeled cRNA were hybridized on Agilent “Mouse Gene Expression 8x60K Microarray” (Design ID: 028005), for 17 hrs, at 65°C under 10 rpm.
Scan protocol Following washing, the slides were scanned using an Agilent G2565CA microarray Scanner System, at a 3 µm resolution in a 20-bit scan mode, according to the “AgilentG3_GX_1Color” protocol (scan resolution : 5 µm, Tiff : 20 bit).
Description Gene expression in control
Data processing Raw .tif images were then extracted using Agilent “Feature Extraction, version 10.10.1.1” following “GE1_1010_Sep10” protocol.
 
Submission date Jun 18, 2019
Last update date Aug 14, 2019
Contact name Gérard Gradwohl
E-mail(s) gradwohl@igbmc.fr
Organization name IGBMC
Street address 1 rue Laurent Fries
City Illkirch-Graffenstaden
State/province -
ZIP/Postal code 67400
Country France
 
Platform ID GPL13912
Series (2)
GSE132919 Identification of Rfx6-regulated genes in mouse embryonic small intestine endocrine cells by microarrays
GSE133038 Rfx6-regulated genes in the mouse intestine

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
4 59.5625
5 95.25
6 63.6875
7 74
8 3219.81
9 69.375
10 73
11 85.375
12 57.4375
13 363.313
14 4341.63
15 108.625
16 65.375
17 3989.31
18 255.875
19 3699.06
20 44195.5
21 417.625
22 396.063
23 1817.81

Total number of rows: 62969

Table truncated, full table size 807 Kbytes.




Supplementary file Size Download File type/resource
GSM3896355_252800511808_A11087_S01_GE1_1010_Sep10_2_3.txt.gz 12.1 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap