|
| Status |
Public on Oct 06, 2009 |
| Title |
3857_Stom_Tum_U133A |
| Sample type |
RNA |
| |
|
| Source name |
primary gastric tumor, 3857
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: gastric tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini spin columns (Qiagen) were used according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 to 5 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Human Genome U133 Array Set HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000 7G.
|
| Description |
Series of 31 primary gastric tumors
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 30, 2009 |
| Last update date |
Oct 06, 2009 |
| Contact name |
Chia-Huey Ooi |
| Organization name |
F. Hoffmann-La Roche Ltd.
|
| Street address |
Grenzacherstrasse 124
|
| City |
Basel |
| ZIP/Postal code |
4070 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE15456 |
Primary Gastric Cancer Expression Profiles (UK Patient Cohort) |
| GSE15460 |
Oncogenic Pathway Combinations Predict Clinical Prognosis in Gastric Cancer |
|
