|
| Status |
Public on Oct 06, 2009 |
| Title |
MKN45(2) |
| Sample type |
RNA |
| |
|
| Source name |
gastric cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MKN45
cell type: gastric cancer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini spin columns (Qiagen) were used according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000 7G.
|
| Description |
Series of 25 (unique) gastric cancer cell lines
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 30, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Chia-Huey Ooi |
| Organization name |
F. Hoffmann-La Roche Ltd.
|
| Street address |
Grenzacherstrasse 124
|
| City |
Basel |
| ZIP/Postal code |
4070 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE15455 |
GEMINI (Gastric Encyclopedia of Molecular Interactions and Nodes for Intervention) Phases A-C |
| GSE15460 |
Oncogenic Pathway Combinations Predict Clinical Prognosis in Gastric Cancer |
|
| Relations |
| Reanalyzed by |
GSE119087 |
