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Sample GSM3861682 Query DataSets for GSM3861682
Status Public on Apr 27, 2020
Title Healthy individual 1
Sample type SRA
 
Source name iPSC-CM
Organism Homo sapiens
Characteristics cell type: iPSC-CM
cell origin: Human
Growth protocol Cardiogenic differentiation of human iPSC was performed using a chemically defined protocol using activator and inhibitors of the Wnt signaling pathway.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Direct-zol RNA MiniPrep Plus (Zymo Research) in accordance to manufacturer’s instructions, followed by DNAse treatment.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description raw.counts.txt.gz [Non-CHD1]
Data processing TrimGalore
STAR
featureCounts
Genome_build: hg19
Supplementary_files_format_and_content: raw read counts
 
Submission date Jun 07, 2019
Last update date Apr 27, 2020
Contact name Lei Tian
E-mail(s) tianlei@stanford.edu
Phone 6502388262
Organization name Stanford's Postdoctoral Scholar programs
Street address 1215 Welch Rd
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL24676
Series (1)
GSE132401 RNA-sequencing Analysis Reveals Utility and Limitations of iPSC-derived Cardiomyocytes from Congenital Heart Disease Patients
Relations
BioSample SAMN11977092
SRA SRX5987840

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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