|
| Status |
Public on Apr 27, 2020 |
| Title |
Tetralogy of Fallot 5 |
| Sample type |
SRA |
| |
|
| Source name |
iPSC-CM
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC-CM
cell origin: Human
|
| Growth protocol |
Cardiogenic differentiation of human iPSC was performed using a chemically defined protocol using activator and inhibitors of the Wnt signaling pathway.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Direct-zol RNA MiniPrep Plus (Zymo Research) in accordance to manufacturer’s instructions, followed by DNAse treatment.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
raw.counts.txt.gz [TOF5]
|
| Data processing |
TrimGalore
STAR
featureCounts
Genome_build: hg19
Supplementary_files_format_and_content: raw read counts
|
| |
|
| Submission date |
Jun 07, 2019 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Lei Tian |
| E-mail(s) |
tianlei@stanford.edu
|
| Phone |
6502388262
|
| Organization name |
Stanford's Postdoctoral Scholar programs
|
| Street address |
1215 Welch Rd
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE132401 |
RNA-sequencing Analysis Reveals Utility and Limitations of iPSC-derived Cardiomyocytes from Congenital Heart Disease Patients |
|
| Relations |
| BioSample |
SAMN11977093 |
| SRA |
SRX5987839 |
