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Sample GSM385562 Query DataSets for GSM385562
Status Public on Sep 23, 2009
Title H1 hESC, rep1
Sample type RNA
 
Source name H1 ESCs
Organism Homo sapiens
Characteristics potency: pluripotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 10h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description replicate 1 of 2.
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Mar 23, 2009
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6883
Series (1)
GSE15355 Direct Reprogramming of Human Neural Stem Cells by OCT4

Data table header descriptions
ID_REF
VALUE Quantile normalized

Data table
ID_REF VALUE
ILMN_1343291 13.791849
ILMN_1651209 4.492365
ILMN_1651228 10.646377
ILMN_1651229 5.779928
ILMN_1651235 2.614366
ILMN_1651236 1.471043
ILMN_1651237 8.156037
ILMN_1651238 1.733759
ILMN_1651254 8.914063
ILMN_1651260 1.783308
ILMN_1651262 12.249823
ILMN_1651268 2.313504
ILMN_1651278 6.212229
ILMN_1651282 4.124750
ILMN_1651285 2.845930
ILMN_1651286 2.778603
ILMN_1651292 2.393209
ILMN_1651303 1.498638
ILMN_1651309 2.035470
ILMN_1651315 7.896780

Total number of rows: 24526

Table truncated, full table size 527 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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