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Sample GSM385557 Query DataSets for GSM385557
Status Public on Sep 23, 2009
Title 1F hNiPS, rep2
Sample type RNA
 
Source name 1F (Oct4) hiPS from hNSC
Organism Homo sapiens
Characteristics potency: pluripotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 10h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description replicate 2 of 2.
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Mar 23, 2009
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6883
Series (1)
GSE15355 Direct Reprogramming of Human Neural Stem Cells by OCT4

Data table header descriptions
ID_REF
VALUE Quantile normalized

Data table
ID_REF VALUE
ILMN_1343291 13.938577
ILMN_1651209 4.609751
ILMN_1651228 9.663630
ILMN_1651229 5.790405
ILMN_1651235 2.181645
ILMN_1651236 2.723467
ILMN_1651237 8.001336
ILMN_1651238 2.829676
ILMN_1651254 9.192912
ILMN_1651260 2.213627
ILMN_1651262 12.513833
ILMN_1651268 2.734078
ILMN_1651278 6.363214
ILMN_1651282 5.236832
ILMN_1651285 3.464306
ILMN_1651286 2.049133
ILMN_1651292 2.088406
ILMN_1651303 0.962155
ILMN_1651309 1.718913
ILMN_1651315 8.484083

Total number of rows: 24526

Table truncated, full table size 527 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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