NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM38361 Query DataSets for GSM38361
Status Public on Oct 04, 2005
Title 18.Woodruff_8sA1.CEL
Sample type RNA
 
Source name isolated alveolar macrophages
Organism Homo sapiens
Characteristics status: Asthmatic
Extracted molecule total RNA
Extraction protocol RNA was extracted from mouse and human alveolar macrophages and DNase-treated using the RNeasy RNA extraction kit (Qiagen). RNA concentration and quality were assessed using the Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
Label protocol Labeling was performed at the UCSF core facility (cat.ucsf.edu) as per Affymetrix's standard labeling protocol for this chip type (www.affymetrix.com)
 
Hybridization protocol Samples were hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChips. RNA was ampli?ed by performing two rounds of in vitro transcription (Ambion Message Amp aRNA kit no. 1750).
Scan protocol Scanning was performed at the UCSF core facility (cat.ucsf.edu) as per Affymetrix's standard scanning protocol for this chip type (www.affymetrix.com)
Description Total RNA from freshly isolated macrophages
Data processing Bioconductor was used for quality control (affyPLM) and preprocessing (RMA).
 
Submission date Jan 04, 2005
Last update date Aug 28, 2018
Contact name Michael E. Salazar
Phone (415) 514-4371
URL http://arrays.ucsf.edu
Organization name University of California, San Francisco
Department
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL570
Series (1)
GSE2125 isolated alveolar macrophages
Relations
Reanalyzed by GSE46903
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA normalized intensity

Data table
ID_REF VALUE
1007_s_at 8.325925
1053_at 8.759062
117_at 8.307058
121_at 11.389198
1255_g_at 5.058008
1294_at 9.61642
1316_at 7.220461
1320_at 6.696779
1405_i_at 5.021901
1431_at 4.95191
1438_at 6.603431
1487_at 8.973582
1494_f_at 7.72684
1552256_a_at 8.422934
1552257_a_at 9.134917
1552258_at 7.097176
1552261_at 6.735346
1552263_at 9.882129
1552264_a_at 9.104343
1552266_at 5.128139

Total number of rows: 54675

Table truncated, full table size 1056 Kbytes.




Supplementary file Size Download File type/resource
GSM38361.CEL.gz 8.5 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap