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Sample GSM3834458 Query DataSets for GSM3834458
Status Public on Jun 19, 2019
Title WT2
Sample type SRA
Source name liver
Organism Mus musculus
Characteristics genotype: wild type
tissue: liver
molecule subtype: mitochondrial enriched RNA
Extracted molecule total RNA
Extraction protocol Mouse liver tissue lysate was enriched for mitochondria. RNA was treated with Ribo Zero Gold and DNase. Bisulfite treatment (Epigentek, three cycles of 90°C for 5 min and 60°C for 1h)
Illumina TruSeq small RNA
Library strategy Bisulfite-Seq
Library source transcriptomic
Library selection RANDOM
Instrument model Illumina MiSeq
Data processing Quality and 3' end adapter trimming with Trim_Galore!
Reads longer than 20nt were aligned to GRCh38 with Bismark
Genome_build: GRCh38
Submission date May 31, 2019
Last update date Jun 26, 2019
Contact name Lindsey Van Haute
Organization name University of Cambridge
Department MRC Mitochondrial Biology Unit
Street address Hills Road
City Cambridge
ZIP/Postal code CB2 0XY
Country United Kingdom
Platform ID GPL16417
Series (1)
GSE132020 NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs
BioSample SAMN11898191
SRA SRX5938163

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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