|
Status |
Public on Mar 20, 2009 |
Title |
Hues 49 |
Sample type |
RNA |
|
|
Source name |
undifferentiated human ES cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: undifferentiated human ES cells development stage: embryonic
|
Treatment protocol |
Chen et al., Cell Stem Cell 4, February 6, 2009, pp103-106
|
Growth protocol |
Standard human embryonic stem cell media.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNAeasy Plus Mini Kit Qiagen
|
Label |
biotin
|
Label protocol |
according to Illumina protocols
|
|
|
Hybridization protocol |
Hybridization according to Illumina protocols on Illumina humanRef-8 v3 BeadArray
|
Scan protocol |
according to Illumina protocols
|
Description |
Chip # 4256425034; Array E
|
Data processing |
Variance stabilization before normalization using the vst function in the illumina package (http://www.bioconductor.org/packages/bioc/vignettes/lumi/inst/doc/lumi.pdf) Quantile normalization, due to normalization, there is no p value.
|
|
|
Submission date |
Mar 19, 2009 |
Last update date |
Mar 19, 2009 |
Contact name |
Dieter Egli |
E-mail(s) |
degli@nyscf.org
|
Phone |
617 4968659
|
Organization name |
The New York Stem Cell Foundation Research Institute
|
Department |
NYSCF
|
Lab |
Egli
|
Street address |
3960 Broadway
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE15294 |
Gene expression of genetically related and unrelated human embryonic stem cell lines |
|