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Sample GSM380913 Query DataSets for GSM380913
Status Public on Mar 17, 2009
Title Chronic Lymphocytic Leukemia (Mutated) CLL M 639
Sample type RNA
 
Channel 1
Source name CLL M 639
Organism Homo sapiens
Characteristics tissue: Frozen Biopsy Specimen; Chronic Lymphocytic Leukemia (Mutated)
Extracted molecule total RNA
Extraction protocol Exiqon
Label Cy5
Label protocol MiRNA expression profiling from human B cell lymphomas was conducted using up to 1 ug of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark).
 
Channel 2
Source name Human Normal Lymph node total RNA (Ambion)
Organism Homo sapiens
Characteristics cell type: Normal lymph node
Extracted molecule total RNA
Extraction protocol Exiqon
Label Cy3
Label protocol MiRNA expression profiling from human B cell lymphomas was conducted using up to 1 ug of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark).
 
 
Hybridization protocol Exiqon protocol; The fluorescently labeled samples were combined and hybridized to a miRNA microarray (v.10.0, Exiqon, Denmark), in a nitrogen atmosphere.
Scan protocol GenePix Pro 6.0.1.25. The microarray slides were scanned with GenePix 4100 Scanner.
Description micro RNA expression
Data processing The GPR file was extracted and exported to Genespring 7.3.1. The raw data were normalized using Data transformation: set measurements less than 0.01 to 0.01, per spot and per chip: Intensity dependent (Lowess) normalization, and per gene: normalize to median
 
Submission date Mar 16, 2009
Last update date Mar 16, 2009
Contact name Dereje D. Jima
E-mail(s) ddjima2014@gmail.com
Phone 9195155932
Organization name North Carolina State University
Department Center for Human Health and the Environment (CHHE) and Bioinformatics Research Center (BRC)
Street address 850 Campus Drive
City Raleigh
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL7722
Series (1)
GSE15250 Patterns of micro RNA expression characterize stages of human B-cell differentiation

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (test/reference)
RATIO normalized ratio (test/reference)

Data table
ID_REF VALUE RATIO
13138
4610 -5.7572 0.018488903
10977 0.2942 1.226208
14266
11102
19603 2.2841 4.8705883
13145
17306
17470
17570
17660
17883
19591
5740 0.1682 1.1236731
10987
11043
14257 -0.1430 0.9056141
11111
11166
13170

Total number of rows: 1277

Table truncated, full table size 15 Kbytes.




Supplementary file Size Download File type/resource
GSM380913.gpr.gz 390.9 Kb (ftp)(http) GPR
Processed data included within Sample table

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