|
Status |
Public on Mar 17, 2009 |
Title |
Chronic Lymphocytic Leukemia (Mutated) CLL M 639 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
CLL M 639
|
Organism |
Homo sapiens |
Characteristics |
tissue: Frozen Biopsy Specimen; Chronic Lymphocytic Leukemia (Mutated)
|
Extracted molecule |
total RNA |
Extraction protocol |
Exiqon
|
Label |
Cy5
|
Label protocol |
MiRNA expression profiling from human B cell lymphomas was conducted using up to 1 ug of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark).
|
|
|
Channel 2 |
Source name |
Human Normal Lymph node total RNA (Ambion)
|
Organism |
Homo sapiens |
Characteristics |
cell type: Normal lymph node
|
Extracted molecule |
total RNA |
Extraction protocol |
Exiqon
|
Label |
Cy3
|
Label protocol |
MiRNA expression profiling from human B cell lymphomas was conducted using up to 1 ug of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark).
|
|
|
|
Hybridization protocol |
Exiqon protocol; The fluorescently labeled samples were combined and hybridized to a miRNA microarray (v.10.0, Exiqon, Denmark), in a nitrogen atmosphere.
|
Scan protocol |
GenePix Pro 6.0.1.25. The microarray slides were scanned with GenePix 4100 Scanner.
|
Description |
micro RNA expression
|
Data processing |
The GPR file was extracted and exported to Genespring 7.3.1. The raw data were normalized using Data transformation: set measurements less than 0.01 to 0.01, per spot and per chip: Intensity dependent (Lowess) normalization, and per gene: normalize to median
|
|
|
Submission date |
Mar 16, 2009 |
Last update date |
Mar 16, 2009 |
Contact name |
Dereje D. Jima |
E-mail(s) |
ddjima2014@gmail.com
|
Phone |
9195155932
|
Organization name |
North Carolina State University
|
Department |
Center for Human Health and the Environment (CHHE) and Bioinformatics Research Center (BRC)
|
Street address |
850 Campus Drive
|
City |
Raleigh |
State/province |
NC |
ZIP/Postal code |
27708 |
Country |
USA |
|
|
Platform ID |
GPL7722 |
Series (1) |
GSE15250 |
Patterns of micro RNA expression characterize stages of human B-cell differentiation |
|