|
| Status |
Public on Mar 11, 2009 |
| Title |
Low Early-Life SES Subject # 748 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
socio-economic status: Low Early-Life SES
cell type: PBMC
|
| Treatment protocol |
Leukocytes were collected by antecubtial venipuncture and PBMC were then isolated by density-gradient centrifugation
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMC were then homogenized in QiaShredder Spin Columns (Qiagen) and the lysates were frozen at -80° C until RNA was extracted using AllPrep DNA/RNA kits (Qiagen; Mississauga, ON).
|
| Label |
biotin
|
| Label protocol |
Used the Illumina TotalPrep RNA Amplification Kit following manufacturer's instructions
|
| |
|
| Hybridization protocol |
RNA was hybridized to Illumina HumanRef-8 v3.0 Expression Beadchips following manufacturer's instructions
|
| Scan protocol |
Chips were scanned on an Illumina Beadstation 500 at the McGill University and Génome Québec Innovation Centre.
|
| Description |
N/A
|
| Data processing |
None - these are raw hybridization intensities, averaged across the 30 bead replicates, provided by Illumina Beadstudio
|
| |
|
| Submission date |
Mar 10, 2009 |
| Last update date |
Mar 10, 2009 |
| Contact name |
Gregory E Miller |
| E-mail(s) |
gemiller@psych.ubc.ca
|
| Organization name |
UBC
|
| Department |
Psychology
|
| Street address |
2136 W Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6L1M4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE15180 |
The Biological Residue of Low Early-Life Social Class |
|
