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Sample GSM378960 Query DataSets for GSM378960
Status Public on Mar 11, 2009
Title Low Early-Life SES Subject # 747
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics socio-economic status: Low Early-Life SES
cell type: PBMC
Treatment protocol Leukocytes were collected by antecubtial venipuncture and PBMC were then isolated by density-gradient centrifugation
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol PBMC were then homogenized in QiaShredder Spin Columns (Qiagen) and the lysates were frozen at -80° C until RNA was extracted using AllPrep DNA/RNA kits (Qiagen; Mississauga, ON).
Label biotin
Label protocol Used the Illumina TotalPrep RNA Amplification Kit following manufacturer's instructions
 
Hybridization protocol RNA was hybridized to Illumina HumanRef-8 v3.0 Expression Beadchips following manufacturer's instructions
Scan protocol Chips were scanned on an Illumina Beadstation 500 at the McGill University and Génome Québec Innovation Centre.
Description N/A
Data processing None - these are raw hybridization intensities, averaged across the 30 bead replicates, provided by Illumina Beadstudio
 
Submission date Mar 10, 2009
Last update date Mar 10, 2009
Contact name Gregory E Miller
E-mail(s) gemiller@psych.ubc.ca
Organization name UBC
Department Psychology
Street address 2136 W Mall
City Vancouver
State/province BC
ZIP/Postal code V6L1M4
Country Canada
 
Platform ID GPL6883
Series (1)
GSE15180 The Biological Residue of Low Early-Life Social Class

Data table header descriptions
ID_REF
VALUE raw average hybridization intensity

Data table
ID_REF VALUE
ILMN_2058782 456.7322
ILMN_2084825 1827.296
ILMN_1796678 1711.957
ILMN_1769388 100.9122
ILMN_1723035 358.365
ILMN_2150856 594.6111
ILMN_1658483 130.4351
ILMN_1720048 821.5924
ILMN_1708323 264.1119
ILMN_1687978 333.3784
ILMN_2159694 2639.964
ILMN_1657234 430.198
ILMN_2353161 84.01735
ILMN_1686116 311.3548
ILMN_2165289 2998.271
ILMN_1776967 161.6744
ILMN_2193213 5137.61
ILMN_2129572 78.63409
ILMN_2102721 2390.469
ILMN_2120022 234.1334

Total number of rows: 16830

Table truncated, full table size 359 Kbytes.




Supplementary data files not provided
Processed data not applicable for this record

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