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Sample GSM378947 Query DataSets for GSM378947
Status Public on Mar 11, 2009
Title High Early-Life SES Subject # 717
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics socio-economic status: High Early-Life SES
cell type: PBMC
Treatment protocol Leukocytes were collected by antecubtial venipuncture and PBMC were then isolated by density-gradient centrifugation
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol PBMC were then homogenized in QiaShredder Spin Columns (Qiagen) and the lysates were frozen at -80° C until RNA was extracted using AllPrep DNA/RNA kits (Qiagen; Mississauga, ON).
Label biotin
Label protocol Used the Illumina TotalPrep RNA Amplification Kit following manufacturer's instructions
 
Hybridization protocol RNA was hybridized to Illumina HumanRef-8 v3.0 Expression Beadchips following manufacturer's instructions
Scan protocol Chips were scanned on an Illumina Beadstation 500 at the McGill University and Génome Québec Innovation Centre.
Description N/A
Data processing None - these are raw hybridization intensities, averaged across the 30 bead replicates, provided by Illumina Beadstudio
 
Submission date Mar 10, 2009
Last update date Mar 10, 2009
Contact name Gregory E Miller
E-mail(s) gemiller@psych.ubc.ca
Organization name UBC
Department Psychology
Street address 2136 W Mall
City Vancouver
State/province BC
ZIP/Postal code V6L1M4
Country Canada
 
Platform ID GPL6883
Series (1)
GSE15180 The Biological Residue of Low Early-Life Social Class

Data table header descriptions
ID_REF
VALUE raw average hybridization intensity

Data table
ID_REF VALUE
ILMN_2058782 91.70233
ILMN_2084825 1284.244
ILMN_1796678 1067.831
ILMN_1769388 482.7627
ILMN_1723035 842.9924
ILMN_2150856 1265.792
ILMN_1658483 1483.377
ILMN_1720048 113.5776
ILMN_1708323 520.1913
ILMN_1687978 587.9982
ILMN_2159694 3517.578
ILMN_1657234 5583.4
ILMN_2353161 83.95084
ILMN_1686116 2219.109
ILMN_2165289 942.638
ILMN_1776967 642.6201
ILMN_2193213 1763.545
ILMN_2129572 460.8503
ILMN_2102721 873.6786
ILMN_2120022 194.7599

Total number of rows: 16830

Table truncated, full table size 359 Kbytes.




Supplementary data files not provided
Processed data not applicable for this record

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