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Sample GSM3767570 Query DataSets for GSM3767570
Status Public on Feb 26, 2020
Title miR-200 Control 1 - Vehicle
Sample type SRA
 
Source name Keratinocytes
Organism Mus musculus
Characteristics agent: Vehicle
cell type: Keratinocytes
Treatment protocol Treated with vehicle or 3 ug/ml doxycycline
Extracted molecule total RNA
Extraction protocol RNA extracted with Trizol
Poly A selected and then NEB Next Ultra Directional kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description HM_C3_Control1
MiSeq and HiSeq 4000
Data processing Aligned with Bowtie2
Genes counted with Htseq against mm10
For miR-200 samples the Hiseq and Miseq counts were combined
Htseq was loaded into DESeq2 for differential analysis using the default settings
Genome_build: mm10
Supplementary_files_format_and_content: Htseq count files and DESeq output for each experiment
 
Submission date May 14, 2019
Last update date Feb 26, 2020
Contact name Glen Bjerke
E-mail(s) Glen.Bjerke@colorado.edu
Organization name University of Colorado
Department MCDB
Lab Yi Lab
Street address 347 UCB
City Boulder
State/province CO
ZIP/Postal code 80301
Country USA
 
Platform ID GPL21103
Series (1)
GSE131205 RNA-seq on miRNA induced mouse keratinocytes. miR-200b cluster and miR-205.
Relations
BioSample SAMN11642376
SRA SRX5830667

Supplementary file Size Download File type/resource
GSM3767570_HM_C3_Control1.htcount.txt.gz 91.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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