|
Status |
Public on May 07, 2019 |
Title |
WT-4 |
Sample type |
SRA |
|
|
Source name |
Motor cortex
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: Motor cortex age: postnatal day 22 genotype: wild type
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from postnatal day 22 mouse motor cortex tissue using the RNA Extraction from Tissue kit (Kurabo, RT-S2). NEBNext® UltraTM RNA library construction
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
fpkm_genename.txt (G8)
|
Data processing |
Illumina CASAVA1.8 used for base calling. Sequenced reads were trimmed for adaptor sequence, reads containing N > 10% (N represents base that cannot be determined) were removed; remove low quality reads: the quality value of over 50% bases of the read is <=20. Differential expression significant analysis of two conditions/groups by using the DESeq2 R package, while the significant criterion is padj<0.05 Genome_build: mm10 Supplementary_files_format_and_content: tab-delimited text file include FPKM values for each sample
|
|
|
Submission date |
May 06, 2019 |
Last update date |
May 07, 2019 |
Contact name |
Timothy Huang |
E-mail(s) |
thuang@sbpdiscovery.org
|
Phone |
858-646-3100
|
Organization name |
Sanford Burnham Prebys Medical Discovery Institute
|
Lab |
Huang/Xu Lab
|
Street address |
10901 N Torrey Pines Rd.
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE130763 |
Membralin deficiency dysregulates astrocytic glutamate homeostasis leading to ALS-like impairment |
|
Relations |
BioSample |
SAMN11582861 |
SRA |
SRX5797696 |