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Sample GSM3753244 Query DataSets for GSM3753244
Status Public on May 07, 2019
Title WT-2
Sample type SRA
 
Source name Motor cortex
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: Motor cortex
age: postnatal day 22
genotype: wild type
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from postnatal day 22 mouse motor cortex tissue using the RNA Extraction from Tissue kit (Kurabo, RT-S2).
NEBNext® UltraTM RNA library construction
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description fpkm_genename.txt (G3)
Data processing Illumina CASAVA1.8 used for base calling.
Sequenced reads were trimmed for adaptor sequence, reads containing N > 10% (N represents base that cannot be determined) were removed; remove low quality reads: the quality value of over 50% bases of the read is <=20.
Differential expression significant analysis of two conditions/groups by using the DESeq2 R package, while the significant criterion is padj<0.05
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text file include FPKM values for each sample
 
Submission date May 06, 2019
Last update date May 07, 2019
Contact name Timothy Huang
E-mail(s) thuang@sbpdiscovery.org
Phone 858-646-3100
Organization name Sanford Burnham Prebys Medical Discovery Institute
Lab Huang/Xu Lab
Street address 10901 N Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL24247
Series (1)
GSE130763 Membralin deficiency dysregulates astrocytic glutamate homeostasis leading to ALS-like impairment
Relations
BioSample SAMN11582866
SRA SRX5797691

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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