|
| Status |
Public on Jun 04, 2020 |
| Title |
DuvrY-pUvrY |
| Sample type |
SRA |
| |
|
| Source name |
bacteria
|
| Organism |
Aeromonas dhakensis |
| Characteristics |
strain: AAK1
genotype/variation: DuvrY-pUvrY mutant
age: 18hr (overnight culture)
medium: LB
|
| Growth protocol |
18 hours (overnight) cultured at 37oC with shaking under 200rpm
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using RNeasy Protect Bacteria Mini Kit (Qiagen, Cat. No. 74524).
RNA libraries were prepared for sequencing using Ion Total RNA-seq Kit V2 and IonOneTouch 2 system
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Ion Proton system used for basecalling
Mapping and whole transcriptome analysis (TopHat and Bowtie2 software package)
RNA-Seq analysis (Partek software package)
Genes were derived from https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=1156800
Genome_build: ASM31519v1
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ...
|
| |
|
| Submission date |
May 03, 2019 |
| Last update date |
Jun 04, 2020 |
| Contact name |
Yi-Wei Chen |
| E-mail(s) |
yiweichen515@gmail.com
|
| Organization name |
NCKU
|
| Street address |
No.1 University Road
|
| City |
Tainan |
| ZIP/Postal code |
701 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL26623 |
| Series (1) |
| GSE130707 |
RNAseq results of Aeromonas dhakensis AAK1, uvrY mutant and uvrY complement |
|
| Relations |
| BioSample |
SAMN11578655 |
| SRA |
SRX5793969 |
