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Sample GSM3744940 Query DataSets for GSM3744940
Status Public on May 13, 2019
Title E14 mESCs CRISPR/Cas9 Site 9 replicate 1
Sample type SRA
Source name Embryonic stem cells
Organism Mus musculus
Characteristics passages: 18-27
strain: 129/Ola
cell sex: M
Treatment protocol Lipofection of pX CRISPR plasmids with puromycin or GFP selection
Growth protocol Feeder-free LIF conditioned mESC growth medium
Extracted molecule genomic DNA
Extraction protocol Qiagen Dneasy Blood and Tissue Kit
PCR based addition of Illumina sequencing adapters
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina MiSeq
Description E14 mESCs targeted using CRISPR/Cas9 at Site 9 (Prickle2, chr6)
Data processing Illumina MiSeq base calling
Adapter trimming using trim_galore 0.3.1
Mapping and allele reconstruction using CRISPResso 1.0.8
Genome_build: mm9
Supplementary_files_format_and_content: CRISPResso allele frequency file
Submission date May 02, 2019
Last update date May 13, 2019
Contact name Dominic Owens
Organization name University of Oxford
Department MRC WIMM
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DS
Country United Kingdom
Platform ID GPL16417
Series (1)
GSE130621 Mapping of microhomologies at CRISPR/Cas9 deletions
BioSample SAMN11565517
SRA SRX5781508

Supplementary file Size Download File type/resource
GSM3744940_Site9_rep1_Alleles_frequency_table.txt.gz 230.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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