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Sample GSM3734478 Query DataSets for GSM3734478
Status Public on Oct 23, 2019
Title c114416_rnaseq_uziel_22rv1_15: 22RV1_DHT_DMSO rep1
Sample type SRA
 
Source name 22RV1 immortalized prostate cancer cell line
Organism Homo sapiens
Characteristics cell line: 22RV1
tissue: prostate adenocarcinoma cancer cell line
Treatment protocol Cells were pretreated with indicated inhibitors for 45 minutes followed by 24 hr stimulation with 5 nM DHT or EtOH.
Growth protocol 22RV1 cells were grown for 72 hrs in charcoal stripped (steroid free) serum.
Extracted molecule total RNA
Extraction protocol RNA was prepared by Qiazol extraction followed by RNeasy mini column (QIAGEN)
10 ug of total RNA was submitted to poly-A selection to remove ribosomal RNA and reverse transcribed to double-stranded cDNA. The cDNA was eblunt ended, adenylated, and ligated with Illumina indexes.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Data processing Illumina Casava1.8 software used for basecalling
Reads were aligned using the OmicSoft aligner and gene expression quantified based on UCSC gene model.
Differentially expressed genes between treatment conditions detected by DESeq2 with a statistical cut-off (FC>2, p<0.01)
Genome_build: B37
Supplementary_files_format_and_content: FPKM value of all samples
 
Submission date Apr 24, 2019
Last update date Oct 23, 2019
Contact name Xin Lu
E-mail(s) xin.x.lu@abbvie.com
Phone 847-937-7942
Organization name Abbvie
Department Oncology Discovery
Street address 1 North Waukegan Rd
City North Chicago
State/province IL
ZIP/Postal code 60064
Country USA
 
Platform ID GPL21290
Series (2)
GSE130247 Selective Inhibition of the Second Bromodomain of BET Family Maintains Anti-Tumor Efficacy and Improves Tolerability (22RV1 RNA-seq)
GSE130269 Selective Inhibition of the Second Bromodomain of BET Family Maintains Anti-Tumor Efficacy and Improves Tolerability
Relations
BioSample SAMN11490541
SRA SRX5729309

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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