|
| Status |
Public on Apr 20, 2019 |
| Title |
5-tag species mixing |
| Sample type |
SRA |
| |
|
| Source name |
E13.5 mouse embryonic fibroblasts, HEK293T cell line
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
mouse cell type: E13.5 mouse embryonic fibroblasts
human cell line: HEK293T
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10x Genomics Chromium single cell preparation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
5tag
polyA RNA
Cells were encapsulated and library prepared using the 10X Chromium instrument with the Single Cell 3' Reagent Kits (v2 chemistry). BAM files and Gene-barcode matrices were generated using 10X Genomics Cellranger version 3.0.2.
|
| Data processing |
Generation of fastq files via https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest
Alignment to mm10 or hg19 genome build via https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest
Genome_build: mm10 or hg19
Supplementary_files_format_and_content: barcodes.tsv, genes.tsv, matrix.mtx
|
| |
|
| Submission date |
Apr 19, 2019 |
| Last update date |
Apr 20, 2019 |
| Contact name |
Samantha Morris |
| E-mail(s) |
s.morris@wustl.edu
|
| Phone |
3147478618
|
| Organization name |
Washington University in St Louis
|
| Street address |
Department of Developmental Biology, Washington University School of
|
| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL22245 |
| Series (1) |
| GSE130065 |
CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics |
|
| Relations |
| BioSample |
SAMN11466693 |
| SRA |
SRX5712376 |
