|Public on Jul 27, 2020
|cell line: A549
infection: Newcastle Disease Virus (NDV)
|Transfection: Cells were transfected with miR-NC1 or miR-30e mimic using Lipofectamine 2000 reagent.
Infection: Cell monolayer were washed with PBS and infected with 5 MOI of NDV virus for 1 hour in serum free DMEM. Cells were washed again with PBS and cultured in DMEM supplemented with 1% FBS after infection.
|A549 cells were cultured in DMEM media supplemented with 10% FBS in 100 mm cell culture dishes. For transfection and infection cells were cultures in a 6 well cell culture plate.
|Total RNA was isolated with the help of Trizol reagent (Invitrogen)
Library was constructed with the help of Illumina TruSeq library preparation kit v2.
|Illumina NovaSeq 6000
|FastQC was used to assess the quality of reads.
Trimmomatic was used for filtering and trimming of bad quality reads and sequences.
HISAT2 was used to map reads to human genome (hg38).
StringTie was used for transcript assembly and generation of abundance files.
DESeq2 R package was used for Differential Expression analysis and various R packages were used for generation of different plots and heat maps.
Supplementary_files_format_and_content: txt files containg not normalized (raw) read counts for each gene.
|Apr 18, 2019
|Last update date
|Jul 27, 2020
|Indore bypass road
|Effect of miRNA-30e-5p on innate immunity during viral infection.