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Sample GSM372124 Query DataSets for GSM372124
Status Public on Feb 17, 2010
Title Cont-CIS 2
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics disease state: healthy
cell type: PBMC
age: 29 years
gender: F
Treatment protocol Peripheral blood mononuclear cells (PBMC) were separated on ficoll-hypaque gradient
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the TRIzol Reagent (Invitrogen, Carlsbad, CA)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
 
Hybridization protocol cDNA was synthesized, labeled, hybridized to HG-U133A-2 array containing 22,215 gene-transcripts according to Affymetrix (Inc, Santa Clara, CA) protocol
Scan protocol Hewlett Packard, GeneArray-TM scanner G2500A
Description working set34
Data processing Data analysis was performed by Partek Genomics Solution software (www.partek.com ). Expression values were computed from raw CEL files by applying the Robust Multi-Chip Average (RMA) background correction algorithm. The RMA correction included: 1) values background correction; 2) quantile normalization; 3) log2 transformation; 4) median polish summarization. In order to avoid the noise caused by variable set effects we normalized each set to pre-saved distribution pattern of a well balanced set used as a reference distribution.
 
Submission date Feb 18, 2009
Last update date Feb 19, 2009
Contact name Michael Gurevich
E-mail(s) Michael.Gurevich@sheba.health.gov.il
Organization name Sheba Medical Center
Department Multiple Sclerosis Center
Lab Neurogenomics and Neuroimmunology
Street address Tel Hashomer
City Ramat Gan
ZIP/Postal code 52621
Country Israel
 
Platform ID GPL571
Series (1)
GSE14895 The trait of MS: Altered transcription regulation of nuclear receptors networks operate in the pre-disease state

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1007_s_at 5.14125
1053_at 5.98957
117_at 7.16399
121_at 6.61359
1255_g_at 2.67713
1294_at 8.13371
1316_at 4.75359
1320_at 3.39758
1405_i_at 11.3017
1431_at 3.22899
1438_at 3.84086
1487_at 6.29542
1494_f_at 3.64048
1598_g_at 6.82553
160020_at 5.9258
1729_at 8.06542
1773_at 5.47518
177_at 4.0968
179_at 7.0112
1861_at 4.96019

Total number of rows: 22215

Table truncated, full table size 407 Kbytes.




Supplementary file Size Download File type/resource
GSM372124.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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