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Sample GSM372121 Query DataSets for GSM372121
Status Public on Feb 17, 2010
Title Cont-CIS 1
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics disease state: healthy
cell type: PBMC
age: 29 years
gender: M
Treatment protocol Peripheral blood mononuclear cells (PBMC) were separated on ficoll-hypaque gradient
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the TRIzol Reagent (Invitrogen, Carlsbad, CA)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
 
Hybridization protocol cDNA was synthesized, labeled, hybridized to HG-U133A-2 array containing 22,215 gene-transcripts according to Affymetrix (Inc, Santa Clara, CA) protocol
Scan protocol Hewlett Packard, GeneArray-TM scanner G2500A
Description working set34
Data processing Data analysis was performed by Partek Genomics Solution software (www.partek.com ). Expression values were computed from raw CEL files by applying the Robust Multi-Chip Average (RMA) background correction algorithm. The RMA correction included: 1) values background correction; 2) quantile normalization; 3) log2 transformation; 4) median polish summarization. In order to avoid the noise caused by variable set effects we normalized each set to pre-saved distribution pattern of a well balanced set used as a reference distribution.
 
Submission date Feb 18, 2009
Last update date Feb 19, 2009
Contact name Michael Gurevich
E-mail(s) Michael.Gurevich@sheba.health.gov.il
Organization name Sheba Medical Center
Department Multiple Sclerosis Center
Lab Neurogenomics and Neuroimmunology
Street address Tel Hashomer
City Ramat Gan
ZIP/Postal code 52621
Country Israel
 
Platform ID GPL571
Series (1)
GSE14895 The trait of MS: Altered transcription regulation of nuclear receptors networks operate in the pre-disease state

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1007_s_at 5.87163
1053_at 5.9233
117_at 7.22346
121_at 6.84777
1255_g_at 2.8733
1294_at 8.13371
1316_at 4.62439
1320_at 3.29078
1405_i_at 10.8408
1431_at 3.00568
1438_at 3.98548
1487_at 6.39385
1494_f_at 3.71057
1598_g_at 6.89985
160020_at 6.41021
1729_at 8.03263
1773_at 5.94763
177_at 3.97256
179_at 7.20776
1861_at 4.82447

Total number of rows: 22215

Table truncated, full table size 408 Kbytes.




Supplementary file Size Download File type/resource
GSM372121.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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