GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM372114 Query DataSets for GSM372114
Status Public on Feb 17, 2010
Title MSf 5
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics disease state: multiple sclerosis free
cell type: PBMC
age: 20 years
gender: F
Treatment protocol Peripheral blood mononuclear cells (PBMC) were separated on ficoll-hypaque gradient
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the TRIzol Reagent (Invitrogen, Carlsbad, CA)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
Hybridization protocol cDNA was synthesized, labeled, hybridized to HG-U133A-2 array containing 22,215 gene-transcripts according to Affymetrix (Inc, Santa Clara, CA) protocol
Scan protocol Hewlett Packard, GeneArray-TM scanner G2500A
Description working set74
Data processing Data analysis was performed by Partek Genomics Solution software ( ). Expression values were computed from raw CEL files by applying the Robust Multi-Chip Average (RMA) background correction algorithm. The RMA correction included: 1) values background correction; 2) quantile normalization; 3) log2 transformation; 4) median polish summarization. In order to avoid the noise caused by variable set effects we normalized each set to pre-saved distribution pattern of a well balanced set used as a reference distribution.
Submission date Feb 18, 2009
Last update date Feb 19, 2009
Contact name Michael Gurevich
Organization name Sheba Medical Center
Department Multiple Sclerosis Center
Lab Neurogenomics and Neuroimmunology
Street address Tel Hashomer
City Ramat Gan
ZIP/Postal code 52621
Country Israel
Platform ID GPL571
Series (1)
GSE14895 The trait of MS: Altered transcription regulation of nuclear receptors networks operate in the pre-disease state

Data table header descriptions
VALUE RMA signal

Data table
1007_s_at 3.47265
1053_at 2.2594
117_at 2.39724
121_at 7.3587
1255_g_at 2.18425
1294_at 2.58435
1316_at 2.76614
1320_at 2.98068
1405_i_at 2.17298
1431_at 2.10809
1438_at 3.19307
1487_at 2.38611
1494_f_at 3.42975
1598_g_at 4.51819
160020_at 4.61497
1729_at 3.27312
1773_at 2.43948
177_at 2.46219
179_at 6.56596
1861_at 2.31209

Total number of rows: 22215

Table truncated, full table size 407 Kbytes.

Supplementary file Size Download File type/resource
GSM372114.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap