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Sample GSM372112 Query DataSets for GSM372112
Status Public on Feb 17, 2010
Title MSf 3
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics disease state: multiple sclerosis free
cell type: PBMC
age: 18 years
gender: M
Treatment protocol Peripheral blood mononuclear cells (PBMC) were separated on ficoll-hypaque gradient
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the TRIzol Reagent (Invitrogen, Carlsbad, CA)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
Hybridization protocol cDNA was synthesized, labeled, hybridized to HG-U133A-2 array containing 22,215 gene-transcripts according to Affymetrix (Inc, Santa Clara, CA) protocol
Scan protocol Hewlett Packard, GeneArray-TM scanner G2500A
Description working set76
Data processing Data analysis was performed by Partek Genomics Solution software ( ). Expression values were computed from raw CEL files by applying the Robust Multi-Chip Average (RMA) background correction algorithm. The RMA correction included: 1) values background correction; 2) quantile normalization; 3) log2 transformation; 4) median polish summarization. In order to avoid the noise caused by variable set effects we normalized each set to pre-saved distribution pattern of a well balanced set used as a reference distribution.
Submission date Feb 18, 2009
Last update date Feb 19, 2009
Contact name Michael Gurevich
Organization name Sheba Medical Center
Department Multiple Sclerosis Center
Lab Neurogenomics and Neuroimmunology
Street address Tel Hashomer
City Ramat Gan
ZIP/Postal code 52621
Country Israel
Platform ID GPL571
Series (1)
GSE14895 The trait of MS: Altered transcription regulation of nuclear receptors networks operate in the pre-disease state

Data table header descriptions
VALUE RMA signal

Data table
1007_s_at 3.16096
1053_at 2.22716
117_at 2.65896
121_at 6.92274
1255_g_at 2.09804
1294_at 2.87564
1316_at 4.55856
1320_at 2.10893
1405_i_at 2.11773
1431_at 1.9307
1438_at 2.82274
1487_at 2.87033
1494_f_at 2.82053
1598_g_at 6.34692
160020_at 4.89962
1729_at 4.36295
1773_at 2.27594
177_at 2.23166
179_at 5.94812
1861_at 2.71975

Total number of rows: 22215

Table truncated, full table size 407 Kbytes.

Supplementary file Size Download File type/resource
GSM372112.CEL.gz 1.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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