GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM3718132 Query DataSets for GSM3718132
Status Public on Jan 19, 2021
Title WT2
Sample type SRA
Source name wild-type_splenic Tfh cells
Organism Mus musculus
Characteristics stain background: C57BL/6J
genotype/variation: wild-type
cell type: splenic Tfh cells
Extracted molecule total RNA
Extraction protocol Tfh cells (SLAM-loCD44+CD62L-CD4+) were directly sorted into TRIzol reagent by a FACSAriaII sorter, and total RNA was extracted using standard Qiagen RNease columns.
RNA libraries were prepared for sequencing using standard Illumina protocols。
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
Data processing The quality of RNA-Seq libraries was assessed by FastQC (v0.11.5).
RNA-Seq libraries were mapped to mouse genome mm10 (version M17) using TopHat (v2.1.1).
Expression levels of all genes were estimated by HTseq (v0.6.1), and Reads Per Kilobase per Million mapped reads (RPKM) counts were calculated.
Genome_build: mm10
Supplementary_files_format_and_content: abundance measurements
Submission date Apr 11, 2019
Last update date Jan 19, 2021
Contact name Shuyang Yu
Organization name China Agricultural University
Department College of Biological Sciences
Street address 2 Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100193
Country China
Platform ID GPL21273
Series (2)
GSE129648 Regulation of Tfh differentiation by Mettl3 [RNA-seq]
GSE129650 m6A methyltransferase METTL3 regulates Tfh cell differentiation
BioSample SAMN11397993
SRA SRX5668321

Supplementary file Size Download File type/resource
GSM3718132_WT_2.geneCounts.txt.gz 184.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap