|
| Status |
Public on Jul 06, 2012 |
| Title |
DTCs (bone marrow aspirates) from Patient 13 (4424556027_D) |
| Sample type |
RNA |
| |
|
| Source name |
DTCs (bone marrow aspirates) from Patient 13
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: disseminated tumour cells
disease: breast cancer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen (Germantown, MD) AllPrep DNA/RNA/Protein extraction kits
|
| Label |
biotin
|
| Label protocol |
Illumina's protocol
|
| |
|
| Hybridization protocol |
Illumina's protocol
|
| Scan protocol |
Illumina's protocol
|
| Description |
n/a
|
| Data processing |
Image data were processed by BeadStudio software using background subtraction and Quantile normalization. The methods to adjust the normalized intensity values see MAQC Consortium’s description [PMID: 16964229]. Log2 expression ratio of an intensity value to the average signal in all samples for each transcript was calculated.
|
| |
|
| Submission date |
Feb 10, 2009 |
| Last update date |
Jul 06, 2012 |
| Contact name |
Dong-Yu Wang |
| Organization name |
UHN-PMH-OCI
|
| Street address |
610 University Ave
|
| City |
Toronto |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE14776 |
Mechanisms and pathways of bone metastasis: challenges and pitfalls of performing molecular research on patient samples |
|
