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Sample GSM3683651 Query DataSets for GSM3683651
Status Public on Mar 01, 2020
Title PHH_1
Sample type SRA
Source name human adult liver tissue
Organism Homo sapiens
Characteristics tissue: human adult liver tissue
cell type: primary human hepatocytes
passage: 0
Extracted molecule total RNA
Extraction protocol Adherent cells and suspension cells were extracted from the blood of tissue samples ,then add lysate TRNzol-A+ ,after lysis ,centrifugation with chloroform ,RNA was extracted from supernatant
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing bcl2fastq2-v2.19.1 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence and low-quality sequence, then mapped to GRCh38.91 whole genome using hisat2-2.1.0 with default parameters
Reads count was calculated by htseq-count with parameters -t exon -f bam -s no -a 10
Genome_build: GRCh38.91
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each Sample
Submission date Mar 22, 2019
Last update date Mar 01, 2020
Contact name wang chuan wen
Organization name AnnoGene
Street address No. 288, Yang Cun road, Chou Jiang Street, Yiwu, Zhejiang
City YiWu
ZIP/Postal code 322000
Country China
Platform ID GPL24676
Series (1)
GSE128717 Human Embryonic Stem Cell-derived Expandable Hepatic Organoids Enable Pathophysiological Model of Alcoholic Liver Injury
BioSample SAMN11233427
SRA SRX5559884

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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