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Sample GSM3683644 Query DataSets for GSM3683644
Status Public on Mar 01, 2020
Title HB_2
Sample type SRA
 
Source name human embryonic stem cells
Organism Homo sapiens
Characteristics tissue: human embryonic stem cells
cell type: hepatoblasts
passage: 0
Extracted molecule total RNA
Extraction protocol Adherent cells and suspension cells were extracted from the blood of tissue samples ,then add lysate TRNzol-A+ ,after lysis ,centrifugation with chloroform ,RNA was extracted from supernatant
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing bcl2fastq2-v2.19.1 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence and low-quality sequence, then mapped to GRCh38.91 whole genome using hisat2-2.1.0 with default parameters
Reads count was calculated by htseq-count with parameters -t exon -f bam -s no -a 10
Genome_build: GRCh38.91
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each Sample
 
Submission date Mar 22, 2019
Last update date Mar 01, 2020
Contact name wang chuan wen
E-mail(s) 729360943@qq.com
Organization name AnnoGene
Street address No. 288, Yang Cun road, Chou Jiang Street, Yiwu, Zhejiang
City YiWu
ZIP/Postal code 322000
Country China
 
Platform ID GPL24676
Series (1)
GSE128717 Human Embryonic Stem Cell-derived Expandable Hepatic Organoids Enable Pathophysiological Model of Alcoholic Liver Injury
Relations
BioSample SAMN11233434
SRA SRX5559877

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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