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Status |
Public on Mar 01, 2020 |
Title |
HS_1 |
Sample type |
SRA |
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Source name |
human embryonic stem cells
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Organism |
Homo sapiens |
Characteristics |
tissue: human embryonic stem cells cell type: hepatic specification passage: 0
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Extracted molecule |
total RNA |
Extraction protocol |
Adherent cells and suspension cells were extracted from the blood of tissue samples ,then add lysate TRNzol-A+ ,after lysis ,centrifugation with chloroform ,RNA was extracted from supernatant RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
bcl2fastq2-v2.19.1 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and low-quality sequence, then mapped to GRCh38.91 whole genome using hisat2-2.1.0 with default parameters Reads count was calculated by htseq-count with parameters -t exon -f bam -s no -a 10 Genome_build: GRCh38.91 Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each Sample
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Submission date |
Mar 22, 2019 |
Last update date |
Mar 01, 2020 |
Contact name |
wang chuan wen |
E-mail(s) |
729360943@qq.com
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Organization name |
AnnoGene
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Street address |
No. 288, Yang Cun road, Chou Jiang Street, Yiwu, Zhejiang
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City |
YiWu |
ZIP/Postal code |
322000 |
Country |
China |
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Platform ID |
GPL24676 |
Series (1) |
GSE128717 |
Human Embryonic Stem Cell-derived Expandable Hepatic Organoids Enable Pathophysiological Model of Alcoholic Liver Injury |
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Relations |
BioSample |
SAMN11233437 |
SRA |
SRX5559874 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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