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Sample GSM3674353 Query DataSets for GSM3674353
Status Public on Mar 01, 2022
Title MDA-MB-231_shC_1
Sample type SRA
 
Source name MDA-MB-231
Organism Homo sapiens
Characteristics cell line: MDA-MB-231
genotype: control
Growth protocol MDA-MB-231 cells were cultured in Leibovitz's L-15 Medium supplemented with 10% FBS and penicillin/streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNA samples were extracted according to RNeasy plus kit (#74104, Qiagen).
Libraries were prepared according to the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Illumina bcl2fastq version 2.19 were used to the basecalling.
Reads was aligned to hg38 by star 2.5.3a with default parameters
Read count was summarized with HTSeq 0.8.0
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
 
Submission date Mar 18, 2019
Last update date Mar 01, 2022
Contact name Liu Quentin
E-mail(s) 18301246519@163.com
Organization name DA LIAN MEDIACAL UNIVERSITY
Street address Lvshunkou District, Dalian, Liaoning
City DA LIAN
ZIP/Postal code 116044
Country China
 
Platform ID GPL24676
Series (1)
GSE128428 Identification of genes are regulated by AURKA at transcription level
Relations
BioSample SAMN11158509
SRA SRX5534184

Supplementary file Size Download File type/resource
GSM3674353_shC_1.txt.gz 2.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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