|
| Status |
Public on Apr 04, 2019 |
| Title |
ASCs from subcutaneous adipose tissue pool 2 |
| Sample type |
RNA |
| |
|
| Source name |
isolated from murine subcutaneous adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Adipose stromal cells
tissue: subcutaneous adipose tissue
gender: male
|
| Treatment protocol |
After mouse sacrifice, adipose tissues were dissected and the stroma vascular fraction was isolated via mechanical and enzymatic digestion. Adipose stromal cells were sorted by Automacs (Miltenyi) according to the immunophenotype CD45-CD31-Sca1+
|
| Growth protocol |
Primary cells: cells are not grown. They are processed just after tissue isolation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2,5 ug total RNA (Encore Biotin Module (NuGEN)).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mo Gene 1.1 ST Array Strip; Affymetrix. Array hybridization, washing and staining were performed as described by the manufacturer (GeneAtlas Hybridization, Wash and Stain Kit for WT Array Strips; Affymetrix) with the GeneAtlas System
|
| Scan protocol |
Arrays were then then scanned (GeneAtlas Imaging Station; Affymetrix) and analyzed by using Command Consol
|
| Description |
primary cell
Gene expression data from ASCs isolated from murine subcutaneous adipose tissue.
|
| Data processing |
The CEL files were imported into Partek Genomic Suite v6.4 (Partek, Inc., MO, USA). Genes with expression > 1.5-fold difference between ScAT and perigonadal (PG) AT (PGAT) originating from ASCs and p<0.05. Cluster analyses and principal component analysis were conducted with Partek default settings
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| |
|
| Submission date |
Mar 06, 2019 |
| Last update date |
Apr 05, 2019 |
| Contact name |
Amandine GIROUSSE |
| E-mail(s) |
amandine.girousse@inserm.fr
|
| Organization name |
CNRS
|
| Street address |
4 bis avenue Hubert Curien, Bâtiment INCERE stromalab
|
| City |
toulouse |
| ZIP/Postal code |
31100 |
| Country |
France |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE127928 |
Expression data from adipose stromal cells |
|
