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Sample GSM3656734 Query DataSets for GSM3656734
Status Public on Apr 04, 2019
Title ASCs from subcutaneous adipose tissue pool 2
Sample type RNA
 
Source name isolated from murine subcutaneous adipose tissue
Organism Mus musculus
Characteristics cell type: Adipose stromal cells
tissue: subcutaneous adipose tissue
gender: male
Treatment protocol After mouse sacrifice, adipose tissues were dissected and the stroma vascular fraction was isolated via mechanical and enzymatic digestion. Adipose stromal cells were sorted by Automacs (Miltenyi) according to the immunophenotype CD45-CD31-Sca1+
Growth protocol Primary cells: cells are not grown. They are processed just after tissue isolation
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2,5 ug total RNA (Encore Biotin Module (NuGEN)).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mo Gene 1.1 ST Array Strip; Affymetrix. Array hybridization, washing and staining were performed as described by the manufacturer (GeneAtlas Hybridization, Wash and Stain Kit for WT Array Strips; Affymetrix) with the GeneAtlas System
Scan protocol Arrays were then then scanned (GeneAtlas Imaging Station; Affymetrix) and analyzed by using Command Consol
Description primary cell
Gene expression data from ASCs isolated from murine subcutaneous adipose tissue.
Data processing The CEL files were imported into Partek Genomic Suite v6.4 (Partek, Inc., MO, USA). Genes with expression > 1.5-fold difference between ScAT and perigonadal (PG) AT (PGAT) originating from ASCs and p<0.05. Cluster analyses and principal component analysis were conducted with Partek default settings
 
Submission date Mar 06, 2019
Last update date Apr 05, 2019
Contact name Amandine GIROUSSE
E-mail(s) amandine.girousse@inserm.fr
Organization name CNRS
Street address 4 bis avenue Hubert Curien, B√Ętiment INCERE stromalab
City toulouse
ZIP/Postal code 31100
Country France
 
Platform ID GPL11533
Series (1)
GSE127928 Expression data from adipose stromal cells

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
10568202 4.85
10346323 5.14
10346328 318.75
10348817 187.60
10425726 3.37
10380067 6.59
10438358 26.70
10604057 5.12
10583905 175.30
10376021 61.73
10382912 38.34
10369252 32.98
10523281 45.25
10437472 3.26
10534066 2.59
10496629 185.97
10477595 3.70
10428921 3.10
10462484 1.99
10462499 2.15

Total number of rows: 23531

Table truncated, full table size 333 Kbytes.




Supplementary file Size Download File type/resource
GSM3656734_SCAT_2.ga.cel.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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